SITE DIRECTED MUTAGENESES
\sˈa͡ɪt da͡ɪɹˈɛktɪd mjˈuːte͡ɪd͡ʒnˌɛsiːz], \sˈaɪt daɪɹˈɛktɪd mjˈuːteɪdʒnˌɛsiːz], \s_ˈaɪ_t d_aɪ_ɹ_ˈɛ_k_t_ɪ_d m_j_ˈuː_t_eɪ_dʒ_n_ˌɛ_s_iː_z]\
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Mutagenesis where the mutation is caused by in vitro induction directed at a specific site in a DNA molecule. The most common method involves use of a chemically synthesized oligonucleotide mutant which can hybridize with the DNA target molecule. The resulting mismatch-carrying DNA duplex may then be transfected into a bacterial cell line and the mutant strands recovered.
By DataStellar Co., Ltd
Word of the day
Platidiam
- An inorganic water-soluble platinum complex. After undergoing hydrolysis, it reacts DNA produce both intra interstrand crosslinks. These crosslinks appear to impair replication and transcription of DNA. The cytotoxicity cisplatin correlates with cellular arrest in G2 phase cell cycle.